AFLP TEKNII PDF

Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

The results obtained based on the adjusted functions except for the mean CV shown in Figure 3 are presented in Table 3. J Genet Breed The RFLP assay reflects restriction aflo variation spread across the genome, because the use of RFLP markers resulted in the greatest average number of alleles per locus as compared to the other marker systems tested.

Our results suggest that AFLP markers are the best choice for the evaluation of diversity and assessing the genetic relationships between tropical maize inbred lines with high accuracy.

Correlations between genetic distances measured with different markers.

We used the median CV value to calculate the following: AFLP also correlates highly with results obtained using the RFLP system and is a fast and reliable system capable of supporting a multiplex approach not requiring previous knowledge of DNA sequencing.

The loci used were: How to cite this article. Similar patterns were observed for both genetic distance and heterosis. For each specific number of loci or bands used the polymorphic markers were submitted to random samplings with replacement bootstrap samples and genetic distances were obtained for each bootstrap sample Tivang et al.

Codominant markers relate each band to an allele, and therefore the boostrap was applied among locus.

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Barbosa Falp ; Isaias O. Although similar average genetic distance values were obtained for the BR and BR intrapopulation crosses, the BR crosses showed the widest range of genetic distances with all of the four different markers assayed; probably because of the broader genetic base of the BR population.

The choice of the appropriate number of polymorphic loci required for a reliable estimation of genetic distance is influenced by the criteria used, and it appears that the maximum and median CV values are the best choice for evaluating the precision of the genetic tekniii estimates based on molecular marker data sets.

Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands. The SSR markers were promising in terms of the polymorphism and information content revealed, but may involve some additional initial costs associated with primer development.

VI Isozyme variation among races of maize in Bolivia.

In order to compare their relative efficiencies as markers and to find the most suitable marker for maize te,nii studies we evaluated 18 inbred tropical maize lines using a number of different loci as markers. Total genomic DNA was isolated from a bulk of five-week-old leaf tissue taken from 16 plants of each line, then being isolated and purified by the method of Hoisington et al.

Except for the RAPD markers, aflpp the markers correlated genetic distance with single cross performance and heterosis which falp that they could be useful in predicting single cross performance and heterosis in intrapopulation crosses for broad-based populations. Bull Soc Vaud Nat In this method, co-occurrences are divided by the total number of evaluated loci excluding the negative co-occurrences and thus can be interpreted as the proportion of coincidences in relation to the total number of evaluated loci.

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The results obtained in our study showed high agreement between RFLP and AFLP genetic distance estimates, such estimates having also been highly correlated in other studies Russell et al.

For the AFLP method 20 primer combinations were used and binary scored 1 or 0 with each band being considered a locus while for the SSR method 68 polymorphic primers were used with the binary data being converted into a genotypic matrix which was used to identify alleles and their respective loci.

Materials and methods Plant material and DNA isolation Eighteen S 3 afflp inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al. Twknii general method for DNA fingerprinting. Am J Hum Genet Comparisons of different DNA markers for diversity studies in maize Hahn et al.

When performing RAPD analysis, each band was considered as afll locus.

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Nucleic Acids Res The AFLP system presents good levels of precision in its genetic estimates and single crosses prediction. Jaccard similarities were calculated using version 2. The principles and practice of statistics in biological research, 3 rd edn. January 20, ; Accepted: Because the box-plots for each of our groups of trknii were skewed we used the mean, median and maximum CV values to determine the adequate number of polymorphic loci needed for acceptable precision. Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationships among tropical maize inbred lines with high accuracy.